Intracellular localization of recombinant mouse L-histidine decarboxylase in COS7 cells
نویسندگان
چکیده
منابع مشابه
Spectroscopic analysis of recombinant rat histidine decarboxylase.
Mammalian histidine decarboxylases have not been characterized well owing to their low amounts in tissues and instability. We describe here the first spectroscopic characterization of a mammalian histidine decarboxylase, i.e. a recombinant version of the rat enzyme purified from transformed Escherichia coli cultures, with similar kinetic constants to those reported for mammalian histidine decar...
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Mature 53 kDa histidine decarboxylase (HDC) peptide is produced from a precursor 74 kDa peptide. The mechanism of specific cleavage by processing enzyme is unknown. Using the recombinant mouse 74 kDa HDC, we found that porcine pancreatic elastase specifically converted the inactive 74 kDa HDC to its active form of 53 kDa HDC.
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The role of histamine (HA) as a neurotransmitter in the CNS, although proposed a decade ago (Schwartz, 1975), has been only progressively recognized (reviewed by Schwartz et al., 1980, 1982, 1984; Hough and Green, 1984). One of the key pieces of expenmental evidence has been the demonstration that synthesis of the amine in CNS depends on a specific enzyme, L-histidine decarboxylase (HDC) (Schwa...
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Histidine decarboxylase was purified 800-fold from the kidneys of thyroxine-treated mice. The purification procedure included precipitation of protein from a crude supernatant after heating it to 55 degrees C at pH 5.5, fractionation with (NH4)2SO4, phosphocellulose column chromatography, chromatofocusing, DEAE-Sepharose column chromatography, gel filtration on Sephacryl S-300 and preparative p...
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ژورنال
عنوان ژورنال: Japanese Journal of Pharmacology
سال: 1998
ISSN: 0021-5198
DOI: 10.1016/s0021-5198(19)40784-1